[CEPCEB_All] *TOMORROW* IIGB Seminar: Markita Landry 5/2

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You are cordially invited to attend
*Markita Landry*
*UC Berkeley*
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*Date:* Friday, May 2
*                 Time:* 12pm - 1:00 pm
             *Location: *Genomics Auditorium 1102

*DNA-Free Approaches to Plant Bioengineering*

*Abstract: *
DNA-free approaches to plant bioengineering offer many advantages over
plant transgenics. For instance, protein delivery to plants offers many
opportunities for gene editing, transcriptional regulation, and through
direction of protein-protein interactions. However, the delivery of
proteins and ribonucleic acids to plants presents both practical and
analytical challenges. We present a GFP bimolecular fluorescence
complementation- based tool, delivered complementation in planta (DCIP),
which allows for unambiguous and quantitative measurement of protein
delivery in leaves. Using DCIP, we demonstrate cell-penetrating peptide
mediated cytosolic delivery of peptides and recombinant proteins in
Nicotiana benthamiana. We show that DCIP enables measurement of delivery
efficiency and enables functional screening of cell penetrating peptide
efficacies for in-plant protein delivery. Finally, we demonstrate that DCIP
detects cell penetrating peptide mediated delivery of recombinantly
expressed proteins such as mCherry and Lifeact into intact leaves. We also
demonstrate, for the first time, delivery of a recombinant plant
transcription factor, WUSCHEL (AtWUS), in N. benthamiana. RT-qPCR analysis
of AtWUS delivery in Arabidopsis seedlings also suggests delivered WUS can
recapitulate AtWUS-overexpression transcriptional changes. Separately, we
discuss recently-developed strategies for sgRNA delivery to plants to
advance plant genome editing applications. In all, nanoscale delivery of
DNA-free tools for plant bioengineering offer new and powerful approaches
to control plant development and to engineer plant physiology.
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