<div dir="ltr"><div class="gmail_default" style="font-family:tahoma,sans-serif">
<p class="gmail-p1" style="color:rgb(0,0,0);font-size:13px;margin:0px;font-variant-numeric:normal;font-variant-east-asian:normal;font-variant-alternates:normal;font-kerning:auto;font-feature-settings:normal;font-stretch:normal;line-height:normal;font-family:"Helvetica Neue"">Dear Genomics Core Users,</p>
<p class="gmail-p2" style="color:rgb(0,0,0);font-size:13px;margin:0px;font-variant-numeric:normal;font-variant-east-asian:normal;font-variant-alternates:normal;font-kerning:auto;font-feature-settings:normal;font-stretch:normal;line-height:normal;font-family:"Helvetica Neue";min-height:15px"><br></p>
<p class="gmail-p1" style="color:rgb(0,0,0);font-size:13px;margin:0px;font-variant-numeric:normal;font-variant-east-asian:normal;font-variant-alternates:normal;font-kerning:auto;font-feature-settings:normal;font-stretch:normal;line-height:normal;font-family:"Helvetica Neue"">We have recently upgraded our Next Generation Sequencing (NGS) equipment with the addition of the new <a href="https://www.illumina.com/systems/sequencing-platforms/nextseq-1000-2000.html">NextSeq 2000 sequencer</a>.</p>
<p class="gmail-p2" style="color:rgb(0,0,0);font-size:13px;margin:0px;font-variant-numeric:normal;font-variant-east-asian:normal;font-variant-alternates:normal;font-kerning:auto;font-feature-settings:normal;font-stretch:normal;line-height:normal;font-family:"Helvetica Neue";min-height:15px"><br></p>
<p class="gmail-p1" style="color:rgb(0,0,0);font-size:13px;margin:0px;font-variant-numeric:normal;font-variant-east-asian:normal;font-variant-alternates:normal;font-kerning:auto;font-feature-settings:normal;font-stretch:normal;line-height:normal;font-family:"Helvetica Neue"">The NextSeq 2000 Sequencing System is an integrated system that includes instrument hardware for the automated generation of DNA clonal clusters by bridge amplification and sequencing, an on-instrument computer, and integrated control software for sequencing run control and primary analysis. There are three types of <a href="https://www.illumina.com/products/by-type/sequencing-kits/cluster-gen-sequencing-reagents/nextseq-1000-2000-reagents.html">flow cell options</a>, the P1 (100 M reads), P2 (400 M reads), and P3 (1.2 B reads), available with outputs of 27-300 Gb per run. The multiple-run configurations accommodate a wide variety of NGS projects with fast speed. For example, the P3 can generate up to 1.2 billion reads per run, which is nearly three times than the NextSeq 500 high output kit. Unlike the large-scale, multi-lane sequencing system (i.e., NovaSeq 6000) where users have to wait to fill all lanes, the NextSeq 2000 allows researchers to purchase the full flow cell without lane sharing and directly sequence and generate the data after library prep. </p><p class="gmail-p1" style="color:rgb(0,0,0);font-size:13px;margin:0px;font-variant-numeric:normal;font-variant-east-asian:normal;font-variant-alternates:normal;font-kerning:auto;font-feature-settings:normal;font-stretch:normal;line-height:normal;font-family:"Helvetica Neue""><br></p><p class="gmail-p1" style="font-size:13px;margin:0px;font-variant-numeric:normal;font-variant-east-asian:normal;font-variant-alternates:normal;font-kerning:auto;font-feature-settings:normal;font-stretch:normal;line-height:normal;font-family:"Helvetica Neue""><font color="#000000">Please contact us for details: </font><span style="font-family:tahoma,sans-serif;font-size:small"><a href="mailto:gencore@ucr.edu">gencore@ucr.edu</a></span></p><p class="gmail-p1" style="color:rgb(0,0,0);font-size:13px;margin:0px;font-variant-numeric:normal;font-variant-east-asian:normal;font-variant-alternates:normal;font-kerning:auto;font-feature-settings:normal;font-stretch:normal;line-height:normal;font-family:"Helvetica Neue""><br></p><p class="gmail-p1" style="color:rgb(0,0,0);margin:0px;font-variant-numeric:normal;font-variant-east-asian:normal;font-variant-alternates:normal;font-kerning:auto;font-feature-settings:normal;font-stretch:normal;line-height:normal;font-family:"Helvetica Neue""><b style=""><font size="4">NGS services are available @ Genomics Core.</font></b></p><p class="gmail-p1" style="color:rgb(0,0,0);font-size:13px;margin:0px;font-variant-numeric:normal;font-variant-east-asian:normal;font-variant-alternates:normal;font-kerning:auto;font-feature-settings:normal;font-stretch:normal;line-height:normal;font-family:"Helvetica Neue""><br></p><table border="0" cellpadding="0" cellspacing="0" width="732" style="color:rgb(0,0,0);font-size:small;border-collapse:collapse;width:549pt">
<colgroup><col width="106" style="width:79pt">
<col width="84" style="width:63pt">
<col width="86" style="width:64pt">
<col width="96" style="width:72pt">
<col width="87" style="width:66pt">
<col width="91" style="width:68pt">
<col width="90" style="width:68pt">
<col width="92" style="width:69pt">
</colgroup><tbody><tr height="22" style="height:16.71pt">
<td height="22" class="gmail-oa1" width="106" style="height:16.71pt;width:79pt;border:1pt solid rgb(91,155,213);background:rgb(222,235,247);text-align:center;vertical-align:top;padding:3.6pt 7.2pt;color:windowtext;font-size:18pt;font-family:Arial">
<p style="margin-top:0pt;margin-bottom:0pt;margin-left:0in;direction:ltr;unicode-bidi:embed;word-break:normal"></p>
</td>
<td colspan="2" class="gmail-oa2" width="170" style="width:127pt;border:1pt solid rgb(91,155,213);background:rgb(189,215,238);text-align:center;vertical-align:top;padding:3.6pt 7.2pt;color:windowtext;font-family:Arial">
<p style="margin-top:0pt;margin-bottom:0pt;margin-left:0in;direction:ltr;unicode-bidi:embed;word-break:normal"><span style="font-family:Calibri;font-weight:bold">MiSeq</span></p>
</td>
<td colspan="2" class="gmail-oa1" width="183" style="width:138pt;border:1pt solid rgb(91,155,213);background:rgb(222,235,247);text-align:center;vertical-align:top;padding:3.6pt 7.2pt;color:windowtext;font-family:Arial">
<p style="margin-top:0pt;margin-bottom:0pt;margin-left:0in;direction:ltr;unicode-bidi:embed;word-break:normal"><span style="font-family:Calibri;font-weight:bold">NextSeq</span><span style="font-family:Calibri;font-weight:bold">
500</span></p>
</td>
<td colspan="3" class="gmail-oa2" width="273" style="width:205pt;border:1pt solid rgb(91,155,213);background:rgb(189,215,238);text-align:center;vertical-align:top;padding:3.6pt 7.2pt;color:windowtext;font-family:Arial">
<p style="margin-top:0pt;margin-bottom:0pt;margin-left:0in;direction:ltr;unicode-bidi:embed;word-break:normal"><span style="font-family:Calibri;font-weight:bold">NextSeq</span><span style="font-family:Calibri;font-weight:bold">
2000</span></p>
</td>
</tr>
<tr height="22" style="height:16.71pt">
<td height="22" class="gmail-oa4" width="106" style="height:16.71pt;width:79pt;border:1pt solid rgb(91,155,213);background:rgb(222,235,247);vertical-align:top;padding:3.6pt 7.2pt;color:windowtext;font-family:Arial">
<p style="margin-top:0pt;margin-bottom:0pt;margin-left:0in;direction:ltr;unicode-bidi:embed;word-break:normal"><span style="font-family:Calibri;font-weight:bold">Flow Cell</span></p>
</td>
<td class="gmail-oa5" width="84" style="width:63pt;border:1pt solid rgb(91,155,213);background:rgb(189,215,238);vertical-align:top;padding:3.6pt 7.2pt;color:windowtext;font-family:Arial">
<p style="margin-top:0pt;margin-bottom:0pt;margin-left:0in;direction:ltr;unicode-bidi:embed;word-break:normal"><span style="font-family:Calibri">v2</span></p>
</td>
<td class="gmail-oa5" width="86" style="width:64pt;border:1pt solid rgb(91,155,213);background:rgb(189,215,238);vertical-align:top;padding:3.6pt 7.2pt;color:windowtext;font-family:Arial">
<p style="margin-top:0pt;margin-bottom:0pt;margin-left:0in;direction:ltr;unicode-bidi:embed;word-break:normal"><span style="font-family:Calibri">v3</span></p>
</td>
<td class="gmail-oa4" width="96" style="width:72pt;border:1pt solid rgb(91,155,213);background:rgb(222,235,247);vertical-align:top;padding:3.6pt 7.2pt;color:windowtext;font-family:Arial">
<p style="margin-top:0pt;margin-bottom:0pt;margin-left:0in;direction:ltr;unicode-bidi:embed;word-break:normal"><span style="font-family:Calibri">Mid</span></p>
</td>
<td class="gmail-oa4" width="87" style="width:66pt;border:1pt solid rgb(91,155,213);background:rgb(222,235,247);vertical-align:top;padding:3.6pt 7.2pt;color:windowtext;font-family:Arial">
<p style="margin-top:0pt;margin-bottom:0pt;margin-left:0in;direction:ltr;unicode-bidi:embed;word-break:normal"><span style="font-family:Calibri">High</span></p>
</td>
<td class="gmail-oa5" width="91" style="width:68pt;border:1pt solid rgb(91,155,213);background:rgb(189,215,238);vertical-align:top;padding:3.6pt 7.2pt;color:windowtext;font-family:Arial">
<p style="margin-top:0pt;margin-bottom:0pt;margin-left:0in;direction:ltr;unicode-bidi:embed;word-break:normal"><span style="font-family:Calibri">P1</span></p>
</td>
<td class="gmail-oa5" width="90" style="width:68pt;border:1pt solid rgb(91,155,213);background:rgb(189,215,238);vertical-align:top;padding:3.6pt 7.2pt;color:windowtext;font-family:Arial">
<p style="margin-top:0pt;margin-bottom:0pt;margin-left:0in;direction:ltr;unicode-bidi:embed;word-break:normal"><span style="font-family:Calibri">P2</span></p>
</td>
<td class="gmail-oa5" width="92" style="width:69pt;border:1pt solid rgb(91,155,213);background:rgb(189,215,238);vertical-align:top;padding:3.6pt 7.2pt;color:windowtext;font-family:Arial">
<p style="margin-top:0pt;margin-bottom:0pt;margin-left:0in;direction:ltr;unicode-bidi:embed;word-break:normal"><span style="font-family:Calibri">P3</span></p>
</td>
</tr>
<tr height="22" style="height:16.71pt">
<td height="22" class="gmail-oa4" width="106" style="height:16.71pt;width:79pt;border:1pt solid rgb(91,155,213);background:rgb(222,235,247);vertical-align:top;padding:3.6pt 7.2pt;color:windowtext;font-family:Arial">
<p style="margin-top:0pt;margin-bottom:0pt;margin-left:0in;direction:ltr;unicode-bidi:embed;word-break:normal"><span style="font-family:Calibri;font-weight:bold">Max Reads</span></p>
</td>
<td class="gmail-oa5" width="84" style="width:63pt;border:1pt solid rgb(91,155,213);background:rgb(189,215,238);vertical-align:top;padding:3.6pt 7.2pt;color:windowtext;font-family:Arial">
<p style="margin-top:0pt;margin-bottom:0pt;margin-left:0in;direction:ltr;unicode-bidi:embed;word-break:normal"><span style="font-family:Calibri">15 M</span></p>
</td>
<td class="gmail-oa5" width="86" style="width:64pt;border:1pt solid rgb(91,155,213);background:rgb(189,215,238);vertical-align:top;padding:3.6pt 7.2pt;color:windowtext;font-family:Arial">
<p style="margin-top:0pt;margin-bottom:0pt;margin-left:0in;direction:ltr;unicode-bidi:embed;word-break:normal"><span style="font-family:Calibri">25 M</span></p>
</td>
<td class="gmail-oa4" width="96" style="width:72pt;border:1pt solid rgb(91,155,213);background:rgb(222,235,247);vertical-align:top;padding:3.6pt 7.2pt;color:windowtext;font-family:Arial">
<p style="margin-top:0pt;margin-bottom:0pt;margin-left:0in;direction:ltr;unicode-bidi:embed;word-break:normal"><span style="font-family:Calibri">130 M</span></p>
</td>
<td class="gmail-oa4" width="87" style="width:66pt;border:1pt solid rgb(91,155,213);background:rgb(222,235,247);vertical-align:top;padding:3.6pt 7.2pt;color:windowtext;font-family:Arial">
<p style="margin-top:0pt;margin-bottom:0pt;margin-left:0in;direction:ltr;unicode-bidi:embed;word-break:normal"><span style="font-family:Calibri">400 M</span></p>
</td>
<td class="gmail-oa5" width="91" style="width:68pt;border:1pt solid rgb(91,155,213);background:rgb(189,215,238);vertical-align:top;padding:3.6pt 7.2pt;color:windowtext;font-family:Arial">
<p style="margin-top:0pt;margin-bottom:0pt;margin-left:0in;direction:ltr;unicode-bidi:embed;word-break:normal"><span style="font-family:Calibri">100 M</span></p>
</td>
<td class="gmail-oa5" width="90" style="width:68pt;border:1pt solid rgb(91,155,213);background:rgb(189,215,238);vertical-align:top;padding:3.6pt 7.2pt;color:windowtext;font-family:Arial">
<p style="margin-top:0pt;margin-bottom:0pt;margin-left:0in;direction:ltr;unicode-bidi:embed;word-break:normal"><span style="font-family:Calibri">400 M</span></p>
</td>
<td class="gmail-oa5" width="92" style="width:69pt;border:1pt solid rgb(91,155,213);background:rgb(189,215,238);vertical-align:top;padding:3.6pt 7.2pt;color:windowtext;font-family:Arial">
<p style="margin-top:0pt;margin-bottom:0pt;margin-left:0in;direction:ltr;unicode-bidi:embed;word-break:normal"><span style="font-family:Calibri">1200 M</span></p>
</td>
</tr>
<tr height="22" style="height:16.71pt">
<td height="22" class="gmail-oa4" width="106" style="height:16.71pt;width:79pt;border:1pt solid rgb(91,155,213);background:rgb(222,235,247);vertical-align:top;padding:3.6pt 7.2pt;color:windowtext;font-family:Arial">
<p style="margin-top:0pt;margin-bottom:0pt;margin-left:0in;direction:ltr;unicode-bidi:embed;word-break:normal"><span style="font-family:Calibri;font-weight:bold">Output</span></p>
</td>
<td class="gmail-oa5" width="84" style="width:63pt;border:1pt solid rgb(91,155,213);background:rgb(189,215,238);vertical-align:top;padding:3.6pt 7.2pt;color:windowtext;font-family:Arial">
<p style="margin-top:0pt;margin-bottom:0pt;margin-left:0in;direction:ltr;unicode-bidi:embed;word-break:normal"><span style="font-family:Calibri">0.7-8.5Gb</span></p>
</td>
<td class="gmail-oa5" width="86" style="width:64pt;border:1pt solid rgb(91,155,213);background:rgb(189,215,238);vertical-align:top;padding:3.6pt 7.2pt;color:windowtext;font-family:Arial">
<p style="margin-top:0pt;margin-bottom:0pt;margin-left:0in;direction:ltr;unicode-bidi:embed;word-break:normal"><span style="font-family:Calibri">3.8-15 Gb</span></p>
</td>
<td class="gmail-oa4" width="96" style="width:72pt;border:1pt solid rgb(91,155,213);background:rgb(222,235,247);vertical-align:top;padding:3.6pt 7.2pt;color:windowtext;font-family:Arial">
<p style="margin-top:0pt;margin-bottom:0pt;margin-left:0in;direction:ltr;unicode-bidi:embed;word-break:normal"><span style="font-family:Calibri">20-29 Gb</span></p>
</td>
<td class="gmail-oa4" width="87" style="width:66pt;border:1pt solid rgb(91,155,213);background:rgb(222,235,247);vertical-align:top;padding:3.6pt 7.2pt;color:windowtext;font-family:Arial">
<p style="margin-top:0pt;margin-bottom:0pt;margin-left:0in;direction:ltr;unicode-bidi:embed;word-break:normal"><span style="font-family:Calibri">30-120 Gb</span></p>
</td>
<td class="gmail-oa5" width="91" style="width:68pt;border:1pt solid rgb(91,155,213);background:rgb(189,215,238);vertical-align:top;padding:3.6pt 7.2pt;color:windowtext;font-family:Arial">
<p style="margin-top:0pt;margin-bottom:0pt;margin-left:0in;direction:ltr;unicode-bidi:embed;word-break:normal"><span style="font-family:Calibri">30 Gb</span></p>
</td>
<td class="gmail-oa5" width="90" style="width:68pt;border:1pt solid rgb(91,155,213);background:rgb(189,215,238);vertical-align:top;padding:3.6pt 7.2pt;color:windowtext;font-family:Arial">
<p style="margin-top:0pt;margin-bottom:0pt;margin-left:0in;direction:ltr;unicode-bidi:embed;word-break:normal"><span style="font-family:Calibri">40-120 Gb</span></p>
</td>
<td class="gmail-oa5" width="92" style="width:69pt;border:1pt solid rgb(91,155,213);background:rgb(189,215,238);vertical-align:top;padding:3.6pt 7.2pt;color:windowtext;font-family:Arial">
<p style="margin-top:0pt;margin-bottom:0pt;margin-left:0in;direction:ltr;unicode-bidi:embed;word-break:normal"><span style="font-family:Calibri">60-360 Gb</span></p>
</td>
</tr>
<tr height="52" style="height:38.99pt">
<td height="52" class="gmail-oa4" width="106" style="height:38.99pt;width:79pt;border:1pt solid rgb(91,155,213);background:rgb(222,235,247);vertical-align:top;padding:3.6pt 7.2pt;color:windowtext;font-family:Arial">
<p style="margin-top:0pt;margin-bottom:0pt;margin-left:0in;direction:ltr;unicode-bidi:embed;word-break:normal"><span style="font-family:Calibri;font-weight:bold">Read length</span></p>
</td>
<td class="gmail-oa5" width="84" style="width:63pt;border:1pt solid rgb(91,155,213);background:rgb(189,215,238);vertical-align:top;padding:3.6pt 7.2pt;color:windowtext;font-family:Arial">
<p style="margin-top:0pt;margin-bottom:0pt;margin-left:0in;direction:ltr;unicode-bidi:embed;word-break:normal"><span style="font-family:Calibri">2x250bp</span></p>
<p style="margin-top:0pt;margin-bottom:0pt;margin-left:0in;direction:ltr;unicode-bidi:embed;word-break:normal"><span style="font-family:Calibri">(more options)</span></p>
</td>
<td class="gmail-oa5" width="86" style="width:64pt;border:1pt solid rgb(91,155,213);background:rgb(189,215,238);vertical-align:top;padding:3.6pt 7.2pt;color:windowtext;font-family:Arial">
<p style="line-height:normal;margin:0pt 0in;text-indent:0in;direction:ltr;unicode-bidi:embed;word-break:normal"><span style="font-family:Calibri">2x300bp</span></p>
<p style="line-height:normal;margin:0pt 0in;text-indent:0in;direction:ltr;unicode-bidi:embed;word-break:normal"><span style="font-family:Calibri">(more options)</span></p>
</td>
<td class="gmail-oa4" width="96" style="width:72pt;border:1pt solid rgb(91,155,213);background:rgb(222,235,247);vertical-align:top;padding:3.6pt 7.2pt;color:windowtext;font-family:Arial">
<p style="line-height:normal;margin:0pt 0in;text-indent:0in;direction:ltr;unicode-bidi:embed;word-break:normal"><span style="font-family:Calibri">2x150bp/</span></p>
<p style="line-height:normal;margin:0pt 0in;text-indent:0in;direction:ltr;unicode-bidi:embed;word-break:normal"><span style="font-family:Calibri">1X150bp</span></p>
</td>
<td class="gmail-oa4" width="87" style="width:66pt;border:1pt solid rgb(91,155,213);background:rgb(222,235,247);vertical-align:top;padding:3.6pt 7.2pt;color:windowtext;font-family:Arial">
<p style="line-height:normal;margin:0pt 0in;text-indent:0in;direction:ltr;unicode-bidi:embed;word-break:normal"><span style="font-family:Calibri">2x150bp/</span></p>
<p style="line-height:normal;margin:0pt 0in;text-indent:0in;direction:ltr;unicode-bidi:embed;word-break:normal"><span style="font-family:Calibri">1x75bp/</span></p>
<p style="line-height:normal;margin:0pt 0in;text-indent:0in;direction:ltr;unicode-bidi:embed;word-break:normal"><span style="font-family:Calibri">2x75bp</span></p>
</td>
<td class="gmail-oa5" width="91" style="width:68pt;border:1pt solid rgb(91,155,213);background:rgb(189,215,238);vertical-align:top;padding:3.6pt 7.2pt;color:windowtext;font-family:Arial">
<p style="line-height:normal;margin:0pt 0in;text-indent:0in;direction:ltr;unicode-bidi:embed;word-break:normal"><span style="font-family:Calibri">See below</span></p>
</td>
<td class="gmail-oa5" width="90" style="width:68pt;border:1pt solid rgb(91,155,213);background:rgb(189,215,238);vertical-align:top;padding:3.6pt 7.2pt;color:windowtext;font-family:Arial">
<p style="line-height:normal;margin:0pt 0in;text-indent:0in;direction:ltr;unicode-bidi:embed;word-break:normal"><span style="font-family:Calibri">See below</span></p>
</td>
<td class="gmail-oa5" width="92" style="width:69pt;border:1pt solid rgb(91,155,213);background:rgb(189,215,238);vertical-align:top;padding:3.6pt 7.2pt;color:windowtext;font-family:Arial">
<p style="line-height:normal;margin:0pt 0in;text-indent:0in;direction:ltr;unicode-bidi:embed;word-break:normal"><span style="font-family:Calibri">See below</span></p></td></tr></tbody></table></div><div><br></div><div><img src="cid:ii_lf77r0hi1" alt="image.png" style="margin-right: 0px;" width="740" height="345"><br><div class="gmail_default" style="font-family:tahoma,sans-serif;font-size:small;color:rgb(0,0,0)"></div><div class="gmail_default" style="font-family:tahoma,sans-serif;font-size:small;color:rgb(0,0,0)"><span style="font-family:"Helvetica Neue";font-size:13px">Best regards,</span></div><p class="gmail-p1" style="font-variant-numeric:normal;font-variant-east-asian:normal;font-variant-alternates:normal;font-kerning:auto;font-feature-settings:normal;font-stretch:normal;font-size:13px;line-height:normal;font-family:"Helvetica Neue";color:rgb(0,0,0);margin:0px"></p><p class="gmail-p1" style="font-variant-numeric:normal;font-variant-east-asian:normal;font-variant-alternates:normal;font-kerning:auto;font-feature-settings:normal;font-stretch:normal;font-size:13px;line-height:normal;font-family:"Helvetica Neue";color:rgb(0,0,0);margin:0px">Wei</p></div><div><br></div><span class="gmail_signature_prefix">-- </span><br><div dir="ltr" class="gmail_signature" data-smartmail="gmail_signature"><div dir="ltr"><div><font color="#20124d" face="arial, sans-serif"><b>Wei Zhang, Ph.D. (she/her)</b></font></div><div><font color="#20124d" face="arial, sans-serif">Academic Coordinator, </font><span style="color:rgb(32,18,77);font-family:arial,sans-serif">Genomics Core</span></div><div><span style="color:rgb(32,18,77);font-family:arial,sans-serif">Institute for Integrative Genome Biology</span></div><div><span style="color:rgb(32,18,77);font-family:arial,sans-serif">Dept of Botany and Plant Sciences</span></div><div><span style="color:rgb(32,18,77);font-family:arial,sans-serif">University of California</span></div><div><span style="color:rgb(32,18,77);font-family:arial,sans-serif">Riverside, CA 92521</span></div><div><span style="color:rgb(32,18,77);font-family:arial,sans-serif">Phone: 1-951-481-6878</span></div></div></div></div>