<div dir="ltr"><div class="gmail-adn gmail-ads" style="border-left:none;padding:0px;display:flex;font-family:"Google Sans",Roboto,RobotoDraft,Helvetica,Arial,sans-serif;font-size:medium"><div class="gmail-gs" style="margin:0px;min-width:0px;padding:0px 0px 20px;width:auto"><div class="gmail-"><div id="gmail-:1e7" class="gmail-ii gmail-gt" style="direction:ltr;margin:8px 0px 0px;padding:0px;font-size:0.875rem;overflow-x:hidden"><div id="gmail-:1e8" class="gmail-a3s gmail-aiL" style="direction:ltr;font-variant-numeric:normal;font-variant-east-asian:normal;font-variant-alternates:normal;font-size-adjust:none;font-kerning:auto;font-feature-settings:normal;font-stretch:normal;font-size:small;line-height:1.5;font-family:Arial,Helvetica,sans-serif;overflow:auto hidden"><div dir="ltr"><div style="font-family:Aptos,Aptos_EmbeddedFont,Aptos_MSFontService,Calibri,Helvetica,sans-serif;direction:ltr;text-align:center;margin:0px;font-size:12pt;color:rgb(0,0,0)"><img src="cid:ii_m7mb0v5d0" alt="image.png" width="757" height="20" class="gmail-CToWUd" style="margin-right: 0px;"><br></div><p style="direction:ltr;text-align:center;line-height:1.2;margin-top:4.2pt;margin-right:48.8pt;margin-bottom:0px"><font face="georgia, serif" color="#073763"><span style="font-size:20pt;font-weight:700">You are cordially </span><span style="font-size:20pt;font-weight:700">invited</span><span style="font-size:20pt;font-weight:700"> to attend</span></font></p><p style="direction:ltr;text-align:center;line-height:1.2;margin-top:4.2pt;margin-right:48.8pt;margin-bottom:0px"><span style="color:black"><span class="gmail_default"><font face="georgia, serif" size="6">Julia Laskin</font></span></span></p><div style="direction:ltr;text-align:center;line-height:1.2;margin:0px 48.8pt 0px 0px"><font face="georgia, serif" color="#073763"><b><span class="gmail_default"></span><span class="gmail_default">Purdue University</span></b></font></div><div style="direction:ltr;text-align:center;line-height:1.2;margin:0px 48.8pt 0px 0px"><font face="georgia, serif" color="#073763"><b><span class="gmail_default"><br></span></b></font></div><div style="direction:ltr;text-align:center;line-height:1.2;margin:0px 48.8pt 0px 0px;color:rgb(23,78,134)"></div><div style="direction:ltr;text-align:center;line-height:1.2;margin:0px 48.8pt 0px 0px;font-size:12pt;color:rgb(23,78,134)"><span style="font-size:small;color:rgb(34,34,34)"></span><img src="cid:ii_m7mb1lyd1" alt="image.png" width="319" height="378" class="gmail-CToWUd gmail-a6T" tabindex="0" style="cursor: pointer; outline: 0px;"><br></div><div style="direction:ltr;text-align:center;line-height:1.2;margin:0px 48.8pt 0px 0px;font-size:12pt;color:rgb(23,78,134)"><br></div><p style="direction:ltr;text-align:center;line-height:1.2;margin:0pt 48.8pt 0pt 0px"><font face="georgia, serif" size="4" color="#073763"><b><span class="gmail_default"></span>Ambient Mass Spectrometry Imaging: Recent Developments and Opportunities</b></font></p><p style="direction:ltr;text-align:center;line-height:1.2;margin-top:0.5pt;margin-right:48.8pt;margin-bottom:0px"><font face="georgia, serif"><span style="font-size:16pt;color:black">     </span><font size="4"><span style="color:black">  </span><span style="color:black"><b> Date:</b> Friday, <span class="gmail_default"></span>F<span class="gmail_default">ebruary 28</span></span></font></font></p><p style="direction:ltr;text-align:center;line-height:1.2;margin-top:0.5pt;margin-right:48.8pt;margin-bottom:0px"><span style="color:black"><font face="georgia, serif" size="4">     <b>Time:</b> 12pm - 1:00 pm</font></span></p><p style="direction:ltr;text-align:center;line-height:1.2;margin-top:0.5pt;margin-right:48.8pt;margin-bottom:0px"><span style="color:black"><font face="georgia, serif" size="4">  <b>    Location: </b>Genomics Auditorium 1102</font></span></p><p style="direction:ltr;text-align:center;line-height:1.2;margin-top:0.5pt;margin-right:48.8pt;margin-bottom:0px"><span style="font-size:12pt;color:black"><font face="georgia, serif"><br></font></span></p><p style="direction:ltr;text-align:center;line-height:1.2;margin-top:0px;margin-right:48.8pt;margin-bottom:0px"><span style="font-size:12pt;color:black"><b><font face="georgia, serif">Abstract:</font></b></span></p><p style="text-align:center;direction:ltr;line-height:1.2;margin-top:0px;margin-right:48.8pt;margin-bottom:0px"><font face="georgia, serif"><font size="4"><span style="color:black"><b><span class="gmail_default"></span></b></span>Mass spectrometry imaging (MSI) is a powerful technique for studying the localization of lipids, metabolites,<span class="gmail_default" style="font-size:small"> </span></font><span style="font-size:large">and proteins in biological samples. We have developed nanospray desorption electrospray ionization (nano-DESI),<span class="gmail_default" style="font-size:small"> </span></span><span style="font-size:large">an ambient ionization technique that enables label-free molecular imaging of biological samples with minimal<span class="gmail_default" style="font-size:small"> </span></span><span style="font-size:large">sample pretreatment. Nano-DESI relies on the localized liquid extraction of analyte molecules from the sample into<span class="gmail_default" style="font-size:small"> </span></span><span style="font-size:large">a liquid bridge formed between two glass capillaries. The extracted analytes are transferred to a mass spectrometer<span class="gmail_default" style="font-size:small"></span></span><span style="font-size:large">inlet and ionized by electrospray ionization. Quantification is performed by adding an internal standard to the<span class="gmail_default" style="font-size:small"> </span></span><span style="font-size:large">solvent and normalizing the signals of endogenous analytes to the<span class="gmail_default" style="font-size:small"> </span>corresponding adduct of the standard. Recent<span class="gmail_default" style="font-size:small"> </span></span><span style="font-size:large">developments of nano-DESI MSI instrumentation have enabled quantitative imaging of lipids, metabolites, and<span class="gmail_default" style="font-size:small"> </span></span><span style="font-size:large">proteins in tissues with high<span class="gmail_default" style="font-size:small"> </span>sensitivity and spatial resolution down to 8-10 microns using finely pulled capillaries.<span class="gmail_default" style="font-size:small"> </span></span><span style="font-size:large">Furthermore, we have developed a microfluidic nano-DESI probe, which greatly simplifies the<span class="gmail_default" style="font-size:small"> </span>experimental setup<span class="gmail_default" style="font-size:small"> </span></span><span style="font-size:large">and demonstrates similar performance to the capillary-based probe. We have enhanced the chemical specificity of<span class="gmail_default" style="font-size:small"> </span></span><span style="font-size:large">nano-DESI MSI experiments by coupling it with ion mobility spectrometry and online photochemical<span class="gmail_default" style="font-size:small"> </span></span><span style="font-size:large">derivatization of the extracted analytes. These complementary approaches have been used for isomer-selective<span class="gmail_default" style="font-size:small"> </span></span><font size="4">imaging of biomolecules in tissue samples. Collectively, these new spatial omics approaches will provide unique<span class="gmail_default" style="font-size:small"> </span></font><span style="font-size:large">insights into the biochemical pathways in biological systems.</span></font></p></div><div class="gmail-yj6qo"></div><div class="gmail-adL"></div></div></div><div class="gmail-WhmR8e" style="clear:both"></div></div></div><div class="gmail-ajx" style="clear:both"></div></div><div class="gmail-gA gmail-gt gmail-acV" style="font-size:0.875rem;padding:0px;width:auto;border-bottom-left-radius:0px;border-bottom-right-radius:0px;border-top:none;margin:0px;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial;font-family:"Google Sans",Roboto,RobotoDraft,Helvetica,Arial,sans-serif"><div class="gmail-gB gmail-xu" style="border-top:0px;padding:0px"><div class="gmail-ip gmail-iq" style="clear:both;margin:0px;padding:16px 0px;border-top:none"><div id="gmail-:1e6"><table class="gmail-cf gmail-wS" role="presentation" style="border-collapse:collapse"><tbody><tr><td class="gmail-amq" style="padding:0px 16px;vertical-align:top;width:44px"><img id="gmail-:mz_11" name="gmail-:mz" src="https://lh3.googleusercontent.com/a/ACg8ocIhgTrpJu6_xu9RjKTEaPWoefUri_b-jenQogkhvfzXv2VN9A=s40-p-mo" class="gmail-ajn gmail-bofPge" style="width: 40px; height: 40px; border-radius: 50%; display: block;"></td><td class="gmail-amr" style="padding:0px;width:1195px"><div class="gmail-nr gmail-wR" style="padding:0px;box-sizing:border-box;border-radius:1px;border:none;margin:0px"><div class="gmail-amn" style="height:auto;padding:0px;display:flex;line-height:20px"><span id="gmail-:1ee" role="link" tabindex="0" class="gmail-ams gmail-bkH" style="border:1px solid rgb(116,119,117);display:inline-flex;font-size:0.875rem;background:none;border-radius:18px;box-sizing:border-box;color:rgb(68,71,70);height:36px;outline:none;padding:0px 16px 0px 12px;min-width:104px;margin-right:8px">Reply</span><span id="gmail-:1ec" role="link" tabindex="0" class="gmail-ams gmail-bkG" style="border:1px solid rgb(116,119,117);display:inline-flex;font-size:0.875rem;background:none;border-radius:18px;box-sizing:border-box;color:rgb(68,71,70);height:36px;outline:none;padding:0px 16px 0px 12px;min-width:104px;margin-right:8px">Forward</span></div></div></td></tr></tbody></table></div></div></div></div></div>