[IIGB_All] *TOMORROW* IIGB Seminar: Julia Laskin

[IIGBadmin IIGBadmin]

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You are cordially invited to attend

Julia Laskin
*Purdue University*

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*Ambient Mass Spectrometry Imaging: Recent Developments and Opportunities*

       * Date:* Friday, February 28

     *Time:* 12pm - 1:00 pm

* Location: *Genomics Auditorium 1102


*Abstract:*

Mass spectrometry imaging (MSI) is a powerful technique for studying the
localization of lipids, metabolites, and proteins in biological samples. We
have developed nanospray desorption electrospray ionization (nano-DESI), an
ambient ionization technique that enables label-free molecular imaging of
biological samples with minimal sample pretreatment. Nano-DESI relies on
the localized liquid extraction of analyte molecules from the sample into a
liquid bridge formed between two glass capillaries. The extracted analytes
are transferred to a mass spectrometerinlet and ionized by electrospray
ionization. Quantification is performed by adding an internal standard to
the solvent and normalizing the signals of endogenous analytes to the
corresponding
adduct of the standard. Recent developments of nano-DESI MSI
instrumentation have enabled quantitative imaging of lipids, metabolites,
and proteins in tissues with high sensitivity and spatial resolution down
to 8-10 microns using finely pulled capillaries. Furthermore, we have
developed a microfluidic nano-DESI probe, which greatly simplifies the
experimental
setup and demonstrates similar performance to the capillary-based probe. We
have enhanced the chemical specificity of nano-DESI MSI experiments by
coupling it with ion mobility spectrometry and online photochemical
derivatization
of the extracted analytes. These complementary approaches have been used
for isomer-selective imaging of biomolecules in tissue samples.
Collectively, these new spatial omics approaches will provide unique insights
into the biochemical pathways in biological systems.
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