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<div style="text-align:center"><span style="color:rgb(255,165,0)">June 2021</span></div>
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<li style="text-align:justify"><font color="#000000">Dr. Jennifer Brophy - Plants3D Friday, noon seminar</font></li>
<li style="text-align:justify"><span style="color:rgb(0,0,0)">Community Member Highlight: Plants3D trainees Alex Valenzuela and Alex Borowsky. Read their sage advice on predoctoral fellowship grant writing.</span></li>
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<h1 style="text-align:center;display:block;margin:0px;padding:0px;color:rgb(3,61,221);font-family:Helvetica;font-size:40px;font-style:normal;font-weight:bold;line-height:150%;letter-spacing:normal"><span style="color:rgb(75,0,130)"><span style="font-size:20px"><strong>Friday, June 11th - noon, Plants3D Seminar</strong></span></span></h1>
<div style="text-align:center"><span style="font-size:18px">"Synthetic genetic circuits to modify plant development."</span></div>
<h1 style="text-align:center;display:block;margin:0px;padding:0px;color:rgb(3,61,221);font-family:Helvetica;font-size:40px;font-style:normal;font-weight:bold;line-height:150%;letter-spacing:normal"><span style="color:rgb(75,0,130)"><span style="font-size:20px"><strong>Dr. Jennifer Brophy<br>
Invited by the Plants3D trainees</strong></span></span></h1>
<div style="text-align:justify">Dr. Jennifer Brophy received her Ph.D. from the Massachusetts Institute of Technology (MIT), where she worked in the group of Dr. Chris Voigt and developed methods to tune gene expression in bacteria species. She did a postdoc in the group of Dr. José Dinneny at Stanford University, where she adapted her studies in bacteria to plants. In 2019, she was awarded a Burroughs Wellcome Fund CASI grant entitled "Engineering organ development using synthetic genetic regulation." This grant will help start her independent research lab. Dr. Jennifer Brophy will join the Stanford University Bioengineering Department as an Assistant Professor in September 2021.</div>
<div style="text-align:center"><br>
Thanks to Alex Borowsky for extending the invitation. <br>
More Plants3D Trainee invites in the fall. </div>
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<h1 style="text-align:center;display:block;margin:0px;padding:0px;color:rgb(3,61,221);font-family:Helvetica;font-size:40px;font-style:normal;font-weight:bold;line-height:150%;letter-spacing:normal"><span style="color:rgb(75,0,130)"><span style="font-size:20px">Plants3D Community Profile</span></span></h1>
<div style="text-align:center"><font color="#4b0082"><span style="font-size:20px"><strong>Plants3D trainees Alex Valenzuela, Ford Foundation Predoctoral Fellowship Awardee and<br>
Alex Borowsky, NIFA Predoctoral Fellowship Awardee<br>
<br>
Sage Advice on Grant Writing</strong></span></font></div>
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<span style="font-size:16px"><strong>Alex Valenzuela,</strong> co-mentored by Linda Walling and Peter Atkinson, received a <strong>Ford Predoctoral Fellowship</strong> and an honorable mention for his NSF-GRFP application.</span></div>
<ol>
<li style="text-align:left"><strong>When did you write the grants? </strong></li>
</ol>
<div style="text-align:left">I applied to both the NSF-GRFP and Ford Predoctoral Fellowship during my second year (Fall 2019). This was after I had taken the BPSC200 series. The courses provided me with a good foundation on grant writing and motivated me to start the process.</div>
<ol>
<li style="text-align:left" value="2"><strong>What lessons did you learn in terms of crafting the grant?</strong></li>
</ol>
<div style="text-align:left">The biggest lesson I learned was to <u>start early</u>. I began writing about 2 months before the application was due and still cut it very close.</div>
<ol>
<li style="text-align:left" value="3"><strong>What were the objectives of the grant, and how did you implement them in the grant?</strong></li>
</ol>
<div style="text-align:left">In the personal statement and statement of previous research, the main objective was to show my commitment to students coming from groups underrepresented in academia. I made sure to talk about my previous experiences as someone from an underrepresented group, experiences working with students from similar backgrounds, and how I would utilize resources at UCR to continue to do so.</div>
<ol>
<li style="text-align:left" value="4"><strong>Did you learn from writing more than one proposal about when to start and how much time it takes, e.g., to write a grant</strong></li>
</ol>
<div style="text-align:left">The application required three 2-3 page essays. I spent about a week on each first draft, then another few weeks editing it with the help of my advisor. The essays need to be very short considering all the information needed, so one of the biggest challenges was choosing what details to keep and what to omit.</div>
<ol>
<li style="text-align:left" value="5"><strong>Did you have to include reference letters, and if so, who did you choose to be your reference (can say UG mentor or UCR faculty, but maybe indicate the number of mentors/faculty)?</strong></li>
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<div style="text-align:left">Both the Ford Fellowship and NSF-GRFP applications required 3 reference letters. For both, I chose my two Co-PIs and my former supervisor from an industry internship.</div>
<ol>
<li style="text-align:left" value="6"><strong>Do you think papers/posters from undergrad or graduate work were important in the completion?</strong></li>
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<div style="text-align:left">The Ford application includes previous papers/presentations in their criteria for selection, so I think they played a role in the decision. However, it was only one of the ~15 sections we were rated on, so I wouldn’t say that someone without previous papers or posters should feel deterred.</div>
<ol>
<li style="text-align:left" value="7"><strong>If you applied more than once, did you receive reviewer comments, and were these helpful?</strong></li>
</ol>
<div style="text-align:left">I applied only once, but some of the comments I received for the Ford application suggested that my application could have been stronger if I <u>included a timeline for my proposed research</u> and more first-author publications under my belt.</div>
<ol>
<li style="text-align:left" value="8"><strong>What is the basic science component of your research proposed in the grant you ultimately got awarded?</strong></li>
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<div style="text-align:left">My proposal looks at how plant resistances influence the secretion of salivary proteins (effectors) in sap-sucking insects.</div>
<ol>
<li style="text-align:left" value="9"><strong>How quickly might your findings be applied (awarded grant)?</strong></li>
</ol>
<div style="text-align:left">It’s hard to say how quickly the main findings can be applied. Still, the tools and techniques developed in the process could be used to enable CRISPR-mediated genome editing in <em>Bemisia tabaci</em> (whiteflies).</div>
<ol>
<li style="text-align:left" value="10"><strong>Did your grant include basic to applied aims (awarded grant)?</strong></li>
</ol>
<p dir="ltr" style="text-align:left;color:rgb(34,34,34);font-size:16px;margin:10px 0px;padding:0px;font-family:Helvetica;line-height:150%">Neither application asked about applied aims.<br>
<br>
<span style="font-size:16px"><strong>Alex Borowsky, </strong>a member of the <a href="https://ucr.us1.list-manage.com/track/click?u=20258b97443c8dea22c17955f&id=d019425920&e=f8eca9c9de" style="color:rgb(0,126,158);font-weight:normal;text-decoration:underline" target="_blank">Bailey-Serres lab</a>,<strong> </strong>recently received a <strong>2021</strong> <strong>NIFA Predoctoral Fellowship</strong> and an honorable mention for his NSF-GRFP application.</span></p>
<ol>
<li style="text-align:left"><strong>When did you write the grants? </strong></li>
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<div style="text-align:left">I applied for every grant I was remotely eligible for! I applied for the NSF GRFP twice, as a senior undergraduate and as a second-year grad student. I also applied unsuccessfully for a Hertz fellowship as a first-year grad student and an NDSEG fellowship as a second year. Then I applied for and got funding from Plants 3D. I was finally successful as a third-year applying for extramural funding with the USDA NIFA Predoctoral Fellowship, which you have to advance to candidacy before applying. One key to success is to submit as many different applications as possible.</div>
<ol>
<li style="text-align:left" value="2"><strong>Did you take the BPSC200A/B series before writing the grant, and did you benefit from the BPSC200A/B series? </strong></li>
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<div style="text-align:left">Yes, these classes were definitely a good opportunity to practice writing. The projects proposed in my grant applications (and my qualifying exam proposal) were all evolutions of my plan for the remainder of my dissertation work.</div>
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<li style="text-align:left" value="3"><strong>What lessons did you learn in terms of crafting the grant?</strong></li>
</ol>
<div style="text-align:left">I've learned a few important lessons: to make sure the significance and novelty of your proposal are clear. These are the things that will get reviewers excited more so than details about your experiments planned. To make sure your aims are fully independent - I’ve been dinged on this by reviewers even when explaining that one doesn’t require data from the other. Even if they could appear to be interdependent, make sure it’s clear you can do them without each other. If in doubt, make the “dubiously dependent” aim Aim #1. If that doesn’t work, reframe the goals of your aims. Especially for the NSF GRFP, where an (often deeply) personal statement is required, identify a theme that highlights your personal background and how that has led to your current goals. You obviously can’t fabricate your experiences, but you can craft how you frame them and the self-reflection included in the proposal.<br>
<br>
Finally, I highly recommend making small, colorful pictographic representations of your aims if you are allowed to include figures - it makes it easier for reviewers to grasp your ideas when their eyes are reading a large number of proposals.</div>
<ol>
<li style="text-align:left" value="4"><strong>What were the objectives of the grant, and how did you implement them in the grant?</strong></li>
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<div style="text-align:left">My most recent grant has three main aims: (1) defining and visualizing gene networks controlling suberin biosynthesis in the rice exodermis; (2) characterization of mutant and reporter lines for rice suberin biosynthesis and regulation mutants to understand the role of suberin in drought tolerance and identity hormonal signaling that influences suberin, and (3) developing tools to engineer spatiotemporal gene expression in plants. </div>
<ol>
<li style="text-align:left" value="5"><strong>Can you say a little bit more about the timing of grant writing? Did you learn from writing more than one proposal about when to start and how much time it takes, e.g., to write a grant?</strong></li>
</ol>
<div style="text-align:left">The best plan is to research what opportunities are available to you every year and what opportunities will become available to you in the next year. I read the RFA for the NIFA grant the year before I was eligible and made a note to check back the next year. It’s important to start at least a month or so in advance, especially if you need to ask for new letters of recommendation. But these proposals can come together pretty quickly if you adapt an older submission for each new one. The NIFA proposal took a while because they ask for the same information as a full grant proposal! Not just a personal and research statement like GRFP - lots of extra lists of available equipment, data management plans, etc.</div>
<ol>
<li style="text-align:left" value="6"><strong>Did you have to include reference letters, and if so, who did you choose to be your reference?</strong></li>
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<div style="text-align:left">Yes, every proposal I’ve submitted has required this. My PI at UCR and my undergraduate research mentor are consistent sources of letters, as well as other UCR faculty that I’ve gotten to work with. I haven’t had a letter from a professor outside my own institutions yet, but these people might be great resources if you have strong relationships. You can also consider professors that can speak to different parts of your experiences and describe different facets of your work. For example, I included a letter from Prof. Loralee Larios, with who I have worked on an outreach project. Finally, you can look at the criteria for the award and emphasize to your letter writers aspects they could try to highlight. Again, these might be different for different letter writers.</div>
<ol>
<li style="text-align:left" value="7"><strong>Do you think papers/posters from undergrad or graduate work were important to get your grant?</strong></li>
</ol>
<div style="text-align:left">Absolutely! A frankly brutal piece of advice I’ve heard: “<u>Reviewers don’t always know how to read, but they do know how to count</u>.” I had a second author paper and a co-first author paper from undergrad, and a middle author preprint from grad school when I was successful. I think it’s possible to be successful with a limited publication record, but that’s easier for undergrads or first-year grad students. It would be best to discuss with your PI. It might be possible for you to tackle a smaller project while first in the lab that can be published quickly to make yourself more competitive for extramural funding in the future. Also, take advantage of putting your work on biorxiv - it can demonstrate that you can publish months before the work is in print. Posters and talks are also super great, and you should present these when you have the opportunity, even when you think you don’t have enough data. You can present a great talk or poster that is mostly background and your ideas and plans. My publications were from undergrad, and so posters and talks were my opportunity to demonstrate progress in my dissertation research lab. The Corteva symposia are great - many of them have student speakers open outside the university hosting. I presented my rotation project once at UC Davis in my first year. Send in abstracts when you find opportunities! Don’t worry about having enough data.</div>
<ol>
<li style="text-align:left" value="8"><strong>If you applied more than once, did you receive reviewer comments, and were these helpful?</strong></li>
</ol>
<div style="text-align:left">Reviewer comments can be demoralizing. Don’t let them get you down! We all tend to fixate on negative feedback, but make sure you read closely and highlight (really, use a highlighter on your reviews) the positive aspects reviewers mentioned. It’s impossible to please everyone all the time, so if you are resubmitting or submitting a similar proposal, don’t worry if one reviewer didn’t like it. If two different reviewers both made the same negative comment, that’s then something you should work on for the next proposal. </div>
<ol>
<li style="text-align:left" value="9"><strong>What is the basic science component of your research proposed in the grant you ultimately got awarded? </strong></li>
</ol>
<div style="text-align:left">The basic science aspects in my proposal were mostly dealing with methods to analyze gene regulatory networks and genetic dissection of gene function.</div>
<ol>
<li style="text-align:left" value="10"><strong>How quickly might your findings be applied (awarded grant)? </strong></li>
</ol>
<div style="text-align:left">I think this could take some time. My findings might inform crop breeding or engineering, but getting those aspects executed could take several years beyond my Ph.D.</div>
<ol>
<li style="text-align:left" value="11"><strong>Did your grant include basic to applied aims?</strong></li>
</ol>
<p dir="ltr" style="text-align:left;color:rgb(34,34,34);font-size:16px;margin:10px 0px;padding:0px;font-family:Helvetica;line-height:150%">The overall goal in my proposal was to inform crop engineering, and I hope to start some engineering efforts before graduating from my Ph.D.<br>
</p>
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<p style="text-align:center;color:rgb(34,34,34);font-size:16px;margin:10px 0px;padding:0px;font-family:Helvetica;line-height:150%"><span style="font-size:12px">On the left Alex Borowsky and the right Alex Valenzuela</span></p>
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<div style="text-align:center"><span style="font-size:20px"><span style="color:rgb(128,0,128)"><strong>Trainee Information and Tips</strong></span></span></div>
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Do you want your research or outreach to be featured on our website or in a newsletter? Do you want changes in your bio? Do you have an idea for the community to share? Do you want to feature your PI or a new paper? <br>
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Please email the Plants3D NRT Program Coordinator, Maureen Hummel, about your accomplishments with "Plants3D product" as your subject.<br>
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Be sure to acknowledge your NSF funding on every abstract, poster, and manuscript as follows:<br>
"Funded by the US National Science Foundation, DBI-1922642".<br>
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<strong>Program Contacts</strong><br>
Julia Bailey-Serres,<br>
University Distinguished Professor,<br>
Botany and Plant Sciences<br>
<a href="mailto:serres@ucr.edu" style="color:rgb(0,126,158);font-weight:normal;text-decoration:underline" target="_blank">serres@ucr.edu</a><br>
*<br>
Ian Wheeldon,<br>
Professor,<br>
Chemical and Environmental Engineering <br>
<a href="mailto:Ian.wheeldon@ucr.edu" style="color:rgb(0,126,158);font-weight:normal;text-decoration:underline" target="_blank">Ian.wheeldon@ucr.edu</a><br>
*<br>
Maureen Hummel,<br>
Program Coordinator <br>
<a href="mailto:maureenh@ucr.edu" style="color:rgb(0,126,158);font-weight:normal;text-decoration:underline" target="_blank">maureenh@ucr.edu</a></div>
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<a class="gmail-m_3244155317162116299mcnButton" title="Plants3D NRT Website" href="https://ucr.us1.list-manage.com/track/click?u=20258b97443c8dea22c17955f&id=68a0151aaa&e=f8eca9c9de" style="font-weight:bold;letter-spacing:-0.5px;line-height:100%;text-align:center;text-decoration:none;color:rgb(255,255,255);display:block" target="_blank">Plants3D NRT Website</a>
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<em>Copyright © 2021 Plants3D NSF NRT </em>DBI-1922642<em>, All rights reserved.</em><br>
<a href="http://plants3d.ucr.edu" target="_blank">plants3d.ucr.edu</a>
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