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--></style></head><body lang="EN-US" link="blue" vlink="purple"><div class="WordSection1"><p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1f497d"> </span></p><p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1f497d"> </span></p><div><div style="border:none;border-top:solid #e1e1e1 1.0pt;padding:3.0pt 0in 0in 0in"><p class="MsoNormal"><b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">From:</span></b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"> The California Botanical Society [mailto:<a href="mailto:membership@calbotsoc.org">membership@calbotsoc.org</a>] <br><b>Sent:</b> Tuesday, December 7, 2021 8:47 AM<br><b>To:</b> Amy Litt <<a href="mailto:amy.litt@ucr.edu">amy.litt@ucr.edu</a>><br><b>Subject:</b> CalBotSoc Speaker December 9th, 7pm PT: Dr. Ryoko Oono</span></p></div></div><p class="MsoNormal"> </p><div align="center"><table class="MsoNormalTable" border="0" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse"><tr><td width="100%" valign="top" style="width:100.0%;padding:0in 0in 0in 0in"><div align="center"><table class="MsoNormalTable" border="0" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse"><tr><td valign="top" style="background:#1c291b;padding:33.75pt 0in 33.75pt 0in"><div align="center"><table class="MsoNormalTable" border="0" cellspacing="0" cellpadding="0" width="0" style="width:6.25in;border-collapse:collapse"><tr><td width="600" valign="top" style="width:6.25in;padding:0in 0in 0in 0in"><div align="center"><table class="MsoNormalTable" border="0" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse"><tr><td valign="top" style="padding:0in 0in 0in 0in"><table class="MsoNormalTable" border="0" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse"><tr><td valign="top" style="padding:6.75pt 6.75pt 6.75pt 6.75pt"><table class="MsoNormalTable" border="0" cellspacing="0" cellpadding="0" align="left" width="100%" style="width:100.0%;border-collapse:collapse"><tr><td valign="top" style="padding:0in 6.75pt 0in 6.75pt"><p class="MsoNormal" align="center" style="text-align:center"><img width="100" id="_x0000_i1025" src="https://mcusercontent.com/c632809fa2b75323f2418bb1e/images/f872d1a5-13be-4538-b39a-e8a80e33d0f7.png"></p></td></tr></table></td></tr></table><p class="MsoNormal"> </p><table class="MsoNormalTable" border="0" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;min-width:100%"><tr><td valign="top" style="padding:6.75pt 0in 0in 0in"><table class="MsoNormalTable" border="0" cellspacing="0" cellpadding="0" align="left" width="100%" style="width:100.0%;border-collapse:collapse"><tr><td width="600" valign="top" style="width:6.25in;padding:0in 0in 0in 0in;max-width:100%;min-width:100%"><table class="MsoNormalTable" border="0" cellspacing="0" cellpadding="0" align="left" width="100%" style="width:100.0%;border-collapse:collapse;word-break:break-word"><tr><td valign="top" style="padding:0in 13.5pt 6.75pt 13.5pt"><p class="MsoNormal" align="center" style="text-align:center;line-height:150%"><span style="font-size:18.0pt;line-height:150%;font-family:"Lucida Sans Unicode",sans-serif;color:honeydew">Botany Speaker Series</span><span style="font-family:"Lucida Sans Unicode",sans-serif;color:honeydew"><br><br></span><span style="font-size:13.5pt;line-height:150%;font-family:"Lucida Sans Unicode",sans-serif;color:honeydew">Upcoming talk:</span><span style="font-family:"Verdana",sans-serif;color:#222222"><br> </span></p><h2 align="center" style="text-align:center"><span style="font-family:"Lucida Sans Unicode",sans-serif;color:honeydew">Ryoko Oono, PhD</span></h2><p class="MsoNormal" align="center" style="text-align:center;line-height:150%"><span style="font-family:"Verdana",sans-serif;color:#222222"><br></span><span style="font-size:13.5pt;line-height:150%;font-family:"Verdana",sans-serif;color:honeydew">This Thursday!<br>December 9th 2021<br>7-8 pm PT</span><span style="font-family:"Verdana",sans-serif;color:#222222"><br><br></span><span style="font-size:18.0pt;line-height:150%;font-family:"Lucida Sans Unicode",sans-serif;color:honeydew">"New methods for community survey and species identification: How amplicon sequencing can be used for conservation<em><b><span style="font-family:"Lucida Sans Unicode",sans-serif">"</span></b></em></span><span style="font-family:"Verdana",sans-serif;color:#222222"></span></p></td></tr></table></td></tr></table></td></tr></table></td></tr></table></div></td></tr></table></div></td></tr><tr><td valign="top" style="background:white;padding:27.0pt 0in 33.75pt 0in;background:cover;background-size:cover" id="templateBody"><div align="center"><table class="MsoNormalTable" border="0" cellspacing="0" cellpadding="0" width="0" style="width:6.25in;border-collapse:collapse;max-width:600px!important"><tr><td width="600" valign="top" style="width:6.25in;padding:0in 0in 0in 0in;max-width:600px!important"><div align="center"><table class="MsoNormalTable" border="0" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;background:cover;background-size:cover"><tr><td valign="top" style="padding:0in 0in 0in 0in;min-width:100%"><table class="MsoNormalTable" border="0" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse"><tr><td valign="top" style="padding:6.75pt 6.75pt 6.75pt 6.75pt;min-width:100%"><table class="MsoNormalTable" border="0" cellspacing="0" cellpadding="0" align="left" width="100%" style="width:100.0%;border-collapse:collapse"><tr><td valign="top" style="padding:0in 6.75pt 0in 6.75pt"><p class="MsoNormal" align="center" style="text-align:center"><img width="564" id="_x0000_i1026" src="https://mcusercontent.com/c632809fa2b75323f2418bb1e/_compresseds/63184e59-9bd9-04bc-4adc-e5863f37739e.jpeg"></p></td></tr></table></td></tr></table><p class="MsoNormal"> </p><table class="MsoNormalTable" border="0" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;min-width:100%"><tr><td valign="top" style="padding:6.75pt 0in 0in 0in"><table class="MsoNormalTable" border="0" cellspacing="0" cellpadding="0" align="left" width="100%" style="width:100.0%;border-collapse:collapse"><tr><td width="600" valign="top" style="width:6.25in;padding:0in 0in 0in 0in;max-width:100%;min-width:100%"><table class="MsoNormalTable" border="0" cellspacing="0" cellpadding="0" align="left" width="100%" style="width:100.0%;border-collapse:collapse;word-break:break-word"><tr><td valign="top" style="padding:0in 13.5pt 6.75pt 13.5pt"><h3 align="center" style="text-align:center"><span style="font-family:"Lucida Sans Unicode",sans-serif;color:black">Talk Description</span></h3><p class="MsoNormal" style="line-height:150%"><span style="font-family:"Helvetica",sans-serif;color:#757575"><br></span><span style="font-family:"Lucida Sans Unicode",sans-serif;color:black">Ecosystem monitoring and restoration projects often utilize community composition and species identification to infer site quality and history. For plants, traditional methods for identifying the species that are already present in an area has been to explore its seedbank - the dormant (potential) plant community in the soil. However, traditional seedbank surveys (i.e., growing out seeds in greenhouses) are imperfect methods for capturing the total community of viable seeds from the field while also requiring significant time and training to complete. We aimed to circumvent these issues through the utilization of molecular methods, specifically amplicon sequencing, which reduces the time and extensive training required by traditional methods, while potentially identifying more plant species at lower cost per soil volume. We compared this new method with a traditional greenhouse seedbank study to compare the efficiency and practicality of each strategy. Our study shows that amplicon sequencing can identify a greater diversity of plant species than traditional seedbank surveys and would provide valuable complementary information useful to land managers and restoration ecologists attempting to understand the historic biodiversity of vascular plants at degraded, developing, or natural sites.The talk will also give an overview of other ecological and conservation questions that can be quickly and efficiently answered by using amplicon sequencing methods.</span><span style="font-family:"Helvetica",sans-serif;color:#757575"><br></span><span style="font-family:"Lucida Sans Unicode",sans-serif;color:black"> </span><span style="font-family:"Helvetica",sans-serif;color:#757575"><br><br></span><span style="font-family:"Helvetica",sans-serif;color:black"> </span><span style="font-family:"Helvetica",sans-serif;color:#757575"> </span></p><h3 align="center" style="text-align:center"><strong><span style="font-size:18.0pt;line-height:150%;font-family:"Lucida Sans Unicode",sans-serif;color:black">Join us this Thursday!</span></strong><span style="font-size:18.0pt;line-height:150%;font-family:"Lucida Sans Unicode",sans-serif;color:black"><br><strong><span style="font-family:"Lucida Sans Unicode",sans-serif">December 9th, 2021</span></strong></span><br><strong><span style="font-size:18.0pt;line-height:150%;font-family:"Lucida Sans Unicode",sans-serif;color:black">7-8 pm PT</span></strong></h3></td></tr></table></td></tr></table></td></tr></table><p class="MsoNormal"> </p><table class="MsoNormalTable" border="0" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;min-width:100%"><tr><td valign="top" style="padding:0in 13.5pt 13.5pt 13.5pt"><div align="center"><table class="MsoNormalTable" border="0" cellspacing="0" cellpadding="0" style="background:#00b6c7;border-collapse:collapse"><tr><td style="padding:13.5pt 13.5pt 13.5pt 13.5pt;border-radius:8px"><p class="MsoNormal" align="center" style="text-align:center"><span style="font-size:13.5pt;font-family:"Verdana",sans-serif"><a href="https://calbotsoc.us7.list-manage.com/track/click?u=c632809fa2b75323f2418bb1e&id=68ec430b97&e=b69ac08598" target="_blank" title="Zoom link"><b><span style="color:white;letter-spacing:-.4pt;text-decoration:none">Zoom link</span></b></a> </span></p></td></tr></table></div></td></tr></table><p class="MsoNormal"> </p><table class="MsoNormalTable" border="0" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse;min-width:100%"><tr><td valign="top" style="padding:6.75pt 0in 0in 0in"><table class="MsoNormalTable" border="0" cellspacing="0" cellpadding="0" align="left" width="100%" style="width:100.0%;border-collapse:collapse"><tr><td width="600" valign="top" style="width:6.25in;padding:0in 0in 0in 0in;max-width:100%;min-width:100%"><table class="MsoNormalTable" border="0" cellspacing="0" cellpadding="0" align="left" width="100%" style="width:100.0%;border-collapse:collapse;word-break:break-word"><tr><td valign="top" style="padding:0in 13.5pt 6.75pt 13.5pt"><h3 align="center" style="text-align:center"><br><span style="color:black">Bio</span></h3><p class="MsoNormal" style="line-height:150%"><span style="font-family:"Helvetica",sans-serif;color:#757575"><br></span><span style="font-family:"Lucida Sans Unicode",sans-serif;color:black">Dr. Ryoko Oono is an Associate Professor in the Ecology, Evolution, and Marine Biology department at the University of California, Santa Barbara. Her work has focused on understanding the ecology and evolution of plant-microbe interactions since her PhD at University of Minnesota. For her postdoctoral work, Prof. Oono expanded her field to include broader community ecology of microbes, particularly fungal endophytes - fungi that live inside plant tissues. This new area required Prof. Oono to sometimes pioneer new methods that were becoming mainstream in microbial ecology, namely high-throughput amplicon sequencing. While rapid amplicon sequencing has become standard in microbiology in the last ten years, this method has been applied less to the study of diverse communities of macro-organisms. While not the be-all, end-all to replace traditional methods, Prof. Oono is working to develop efficient, cost-effective sequencing methods that could help answer questions in ecology that require time-intensive and large data collections.</span><span style="font-family:"Helvetica",sans-serif;color:#757575"><br><br> </span></p><p class="MsoNormal" align="center" style="text-align:center;line-height:150%"><strong><i><span style="font-size:10.5pt;line-height:150%;font-family:"Helvetica",sans-serif;color:black">Talk Zoom link: </span></i></strong><span style="font-size:10.5pt;line-height:150%;font-family:"Helvetica",sans-serif;color:black"><br><em><span style="font-family:"Helvetica",sans-serif"><a href="https://calbotsoc.us7.list-manage.com/track/click?u=c632809fa2b75323f2418bb1e&id=aefbcf0af1&e=b69ac08598" target="_blank"><span style="color:#007c89">https://ucr.zoom.us/j/98594151297?pwd=NXlJQ1YrVW10a2N5d1BnRWVrMVdkZz09</span></a></span></em></span><span style="font-family:"Helvetica",sans-serif;color:#757575"><br> </span></p></td></tr></table></td></tr></table></td></tr></table><p class="MsoNormal"> </p><table class="MsoNormalTable" border="0" cellspacing="0" cellpadding="0" width="100%" style="width:100.0%;border-collapse:collapse"><tr><td valign="top" style="padding:6.75pt 6.75pt 6.75pt 6.75pt"><table class="MsoNormalTable" border="0" cellspacing="0" cellpadding="0" align="left" style="border-collapse:collapse"><tr><td valign="top" style="padding:0in 6.75pt 6.75pt 6.75pt"><p class="MsoNormal" align="center" style="text-align:center"><a href="https://calbotsoc.us7.list-manage.com/track/click?u=c632809fa2b75323f2418bb1e&id=735a70c739&e=b69ac08598" target="_blank" title=""><span style="text-decoration:none"><img border="0" width="564" id="_x0000_i1027" src="https://mcusercontent.com/c632809fa2b75323f2418bb1e/_compresseds/f484b289-e62d-ffc9-3e9b-a11078a4df5f.jpeg"></span></a></p></td></tr><tr><td width="582" valign="top" style="width:423.0pt;padding:0in 6.75pt 0in 6.75pt;word-break:break-word"><h2 align="center" style="text-align:center"><strong><span style="font-family:"Lucida Sans Unicode",sans-serif;color:black">Upcoming dates! </span></strong></h2><p class="MsoNormal" style="line-height:150%"><span style="font-size:13.5pt;line-height:150%;font-family:"Helvetica",sans-serif;color:#757575"> </span></p><p class="MsoNormal" align="center" style="text-align:center;line-height:150%"><strong><u><span style="font-size:15.0pt;line-height:150%;font-family:"Lucida Sans Unicode",sans-serif;color:black;background:yellow">Annetta Carter Research Grant Deadline: </span></u></strong><b><span style="font-size:13.5pt;line-height:150%;font-family:"Lucida Sans Unicode",sans-serif;color:black"><br><strong><span style="font-family:"Lucida Sans Unicode",sans-serif">December 31st, 2021</span></strong></span></b><span style="font-size:13.5pt;line-height:150%;font-family:"Lucida Sans Unicode",sans-serif;color:black"><br>For research in Baja California<br>Info & application at: </span><span style="font-size:13.5pt;line-height:150%;font-family:"Lucida Sans Unicode",sans-serif;color:#757575"><a href="https://calbotsoc.us7.list-manage.com/track/click?u=c632809fa2b75323f2418bb1e&id=40c1a5311b&e=b69ac08598" target="_blank"><span style="color:black">https://calbotsoc.org/grants/#AnnettaCarter</span></a></span><span style="font-size:13.5pt;line-height:150%;font-family:"Helvetica",sans-serif;color:#757575"><br> </span></p><p class="MsoNormal" align="center" style="text-align:center;line-height:150%"><span style="font-size:13.5pt;line-height:150%;font-family:"Helvetica",sans-serif;color:#757575"> </span></p><p class="MsoNormal" align="center" style="text-align:center;line-height:150%"><span style="font-size:13.5pt;line-height:150%;font-family:"Helvetica",sans-serif;color:#757575"><br> </span></p><div><p class="MsoNormal" align="center" style="text-align:center;line-height:150%"><strong><u><span style="font-size:13.5pt;line-height:150%;font-family:"Lucida Sans Unicode",sans-serif;color:black">2022 Botany Speaker Series </span></u></strong><span style="font-size:13.5pt;line-height:150%;font-family:"Lucida Sans Unicode",sans-serif;color:black"><br></span><span style="font-family:"Lucida Sans Unicode",sans-serif;color:black">January 13: Mitchell Coleman, UC Riverside/Tejon Conservancy<br>February 10: TBD<br>March 10: Ryan Buck, San Diego State University</span><span style="font-size:13.5pt;line-height:150%;font-family:"Helvetica",sans-serif;color:black"><br></span><span style="font-size:10.0pt;line-height:150%;font-family:"Lucida Sans Unicode",sans-serif;color:black">.<br>.<br>.</span><span style="font-size:13.5pt;line-height:150%;font-family:"Lucida Sans Unicode",sans-serif;color:black"><br></span><span style="font-family:"Lucida Sans Unicode",sans-serif;color:black">November 10: Phebian Odufuwa, Boise State University</span><span style="font-size:13.5pt;line-height:150%;font-family:"Helvetica",sans-serif;color:#757575"><br></span><span style="font-family:"Lucida Sans Unicode",sans-serif;color:black">December 15: TBD</span><span style="font-size:13.5pt;line-height:150%;font-family:"Helvetica",sans-serif;color:#757575"><br><br> </span></p><p class="MsoNormal" align="center" style="text-align:center;line-height:150%"><strong><span style="font-size:13.0pt;line-height:150%;font-family:"Lucida Sans Unicode",sans-serif;color:black">Are </span></strong><em><b><span style="font-size:13.0pt;line-height:150%;font-family:"Lucida Sans Unicode",sans-serif;color:black">YOU</span></b></em><strong><span style="font-size:13.0pt;line-height:150%;font-family:"Lucida Sans Unicode",sans-serif;color:black"> an early career botanist? Want to give a talk in 2022?</span></strong><b><span style="font-size:13.0pt;line-height:150%;font-family:"Lucida Sans Unicode",sans-serif;color:black"><br><strong><span style="font-family:"Lucida Sans Unicode",sans-serif">Email us at <a href="mailto:membership@calbotsoc.org;%20rlolliff@berkeley.edu?subject=Botany%20Speaker%20Series%20Talk" target="_blank"><span style="color:#007c89;font-weight:normal">membership@calbotsoc.org </span></a></span></strong></span></b><span style="font-size:13.5pt;line-height:150%;font-family:"Helvetica",sans-serif;color:#757575"></span></p><p class="MsoNormal" align="center" style="text-align:center;line-height:150%"><span style="font-size:13.5pt;line-height:150%;font-family:"Helvetica",sans-serif;color:#757575"> </span></p><p class="MsoNormal" align="center" style="text-align:center;line-height:150%"><span style="font-size:13.5pt;line-height:150%;font-family:"Helvetica",sans-serif;color:#757575"> 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